Anti-Dynamin 3 antibody
| Name | Anti-Dynamin 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3458 |
| Prices | $380.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Rat Dynamin 3 aa 623-639 |
| Description | Rabbit Polyclonal |
| Gene | DNM3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot of Dynamin 3 on rat liver extract using ab3458.
ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter regionSections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Dynamin activates NO production in rat renal inner medullary collecting ducts via - Dynamin activates NO production in rat renal inner medullary collecting ducts via
Hyndman KA, Musall JB, Xue J, Pollock JS. Am J Physiol Renal Physiol. 2011 Jul;301(1):F118-24. doi:
Distinct functional effects for dynamin 3 during megakaryocytopoiesis. - Distinct functional effects for dynamin 3 during megakaryocytopoiesis.
Wang W, Gilligan DM, Sun S, Wu X, Reems JA. Stem Cells Dev. 2011 Dec;20(12):2139-51.
Kalirin12 interacts with dynamin. - Kalirin12 interacts with dynamin.
Xin X, Rabiner CA, Mains RE, Eipper BA. BMC Neurosci. 2009 Jun 17;10:61.
Endocytic accessory proteins are functionally distinguished by their differential - Endocytic accessory proteins are functionally distinguished by their differential
Mettlen M, Stoeber M, Loerke D, Antonescu CN, Danuser G, Schmid SL. Mol Biol Cell. 2009 Jul;20(14):3251-60.
Isoform and splice-variant specific functions of dynamin-2 revealed by analysis - Isoform and splice-variant specific functions of dynamin-2 revealed by analysis
Liu YW, Surka MC, Schroeter T, Lukiyanchuk V, Schmid SL. Mol Biol Cell. 2008 Dec;19(12):5347-59.