Anti-E1 Ubiquitin Activating Enzyme antibody
| Name | Anti-E1 Ubiquitin Activating Enzyme antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34711 |
| Prices | $387.00 |
| Sizes | 500 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Rat, Human, Mouse, Rabbit, Dog |
| Antigen | Recombinant fusion protein (Human) - Ubiquitin Activating Enzyme E1 (tagged) |
| Description | Rabbit Polyclonal |
| Gene | UBA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image shows human lung tissue stained with ab34711 at 10µg/ml. In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.
ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-E1 Ubiquitin Activating Enzyme antibody (ab34711) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg/ml per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Functional complexity of the axonal growth cone: a proteomic analysis. - Functional complexity of the axonal growth cone: a proteomic analysis.
Estrada-Bernal A, Sanford SD, Sosa LJ, Simon GC, Hansen KC, Pfenninger KH. PLoS One. 2012;7(2):e31858.
Protein ubiquitination is modulated by O-GlcNAc glycosylation. - Protein ubiquitination is modulated by O-GlcNAc glycosylation.
Guinez C, Mir AM, Dehennaut V, Cacan R, Harduin-Lepers A, Michalski JC, Lefebvre T. FASEB J. 2008 Aug;22(8):2901-11.