Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14204(B)]

• Supplier: Abcam
• Catalog: ab181225
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14204(B)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human E1 Ubiquitin Activating Enzyme aa 1000 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: UBA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14204(B)] (ab181225) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.