Anti-E2F5 antibody - N-terminal (ab176017) at 1/1000 dilution + HepG2 cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labeling E2F5 with ab176017 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of ZR-75-1 cells labeling E2F5 with ab176017 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat anti-Rabbit secondary antibodies were used for the analysis.