Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121241 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) of Human testis tissue sections labelling EAN57 with ab121241 (1/20). Antigen retrieval was by HIER pH6. Immunohistochemical staining shows strong cytoplasmic and nuclear positivity in a subset of cells in seminiferus ducts.
Immunohistochemical staining of paraffin embedded Human duodenum tissue, using 1/35 ab121241, shows strong cytoplasmic positivity in enteroendocrine cells.
![All lanes : Anti-EAN57 antibody (ab121241) at 1/250 dilutionLane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11303_EAN57-Primary-antibodies-ab121241-2.jpg)
All lanes : Anti-EAN57 antibody (ab121241) at 1/250 dilutionLane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)