All lanes : Anti-EB2 antibody [1A3] (ab118409) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY EB2 cDNA Lysates/proteins at 5 µg per lane.
All lanes : Anti-EB2 antibody [1A3] (ab118409) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab118409 (1/100 dilution) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.
HEK293T cells transfected with either EB3 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118409 at 1/100 dilution, and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using ab118409 at 1/100 dilution,(Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometric Analysis of Jurkat cells, using ab118409 at 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).