Anti-EB3 antibody [KT36]
| Name | Anti-EB3 antibody [KT36] |
|---|---|
| Supplier | Abcam |
| Catalog | ab53360 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | KT36 |
| Applications | ELISA ICC/IF ICC/IF WB FC |
| Species Reactivities | Human |
| Antigen | Full length human EB3 fused to a tag, purified from E |
| Description | Rat Monoclonal |
| Gene | MAPRE3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
![Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11342_EB3-Primary-antibodies-ab53360-4.jpg)
Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11343_EB3-Primary-antibodies-ab53360-5.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by - Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by
Patel DM, Dubash AD, Kreitzer G, Green KJ. J Cell Biol. 2014 Sep 15;206(6):779-97.
A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at - A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at
Asanov A, Sherry R, Sampieri A, Vaca L. Cell Calcium. 2013 Sep;54(3):246-56.