IHC image of ECH1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153720, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-ECH1 antibody (ab153720) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Anti-ECH1 antibody (ab153720) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Confocal immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECH1 with ab153720 at 1/500 dilution (green). Alpha-tubulin filaments labeled in red.