Anti-ECH1 antibody [9D11AF3]
| Name | Anti-ECH1 antibody [9D11AF3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110294 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 9D11AF3 |
| Applications | ICC/IF ICC/IF ELISA FC IP |
| Species Reactivities | Rat, Human |
| Antigen | human liver mitochondria |
| Description | Mouse Monoclonal |
| Gene | ECH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HL-60 cells were stained with 1 µg/mL ab110294 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Immunocytochemistry image of ab110294 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (9D11AF3, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the mitochondria.