Anti-ECH1 antibody [EPR15449(B)] - C-terminal

• Supplier: Abcam
• Catalog: ab189255
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15449(B)
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 300 to the C-terminus (C terminal)
• Description: Rabbit Monoclonal
• Gene: ECH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-ECH1 antibody [EPR15449(B)] - C-terminal (ab189255) at 1/50000 dilutionLane 1 : Human fetal liver lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunhistochemical analysis of paraffin-embedded human endometrium tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunhistochemical analysis of paraffin-embedded human kidney tissue labeling ECH1 with ab189254 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunfluorescent analysis of 100% methanol(-20℃)-fixed MCF-7 cells labeling ECH1 with ab189254 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling ECH1 with ab189255 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot analysis of ECH1 in human fetal liver lysate immunoprecipitated using ab189254 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.