Immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECHS1 with ab153732 at 1/200 dilution. Right cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue, labeling ECHS1 with ab153732 at 1/250 dilution.
All lanes : Anti-ECHS1 antibody (ab153732) at 1/1000 dilutionLane 1 : Neuro2A whole cell lysateLane 2 : GL261 whole cell lysateLane 3 : C8D30 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-ECHS1 antibody (ab153732) at 1/3000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : PC3 whole cell lysateLane 3 : U-87 MG whole cell lysateLane 4 : SK N SH whole cell lysateLysates/proteins at 30 µg per lane.