All lanes : Anti-EDG2 antibody [EPR9710] (ab166903) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : Caco 2 cell lysateLane 4 : A375 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP IgG antibody at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 using ab166903 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 using ab166903 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.