All lanes : Anti-EDIL3 antibody [EPR12451] (ab190692) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HUVEC cell lysateLane 3 : BxPC-3 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-EDIL3 antibody [EPR12451] (ab190692) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent staining of 4% paraformaldehyde fixed A431 cells labeling EDIL3 with ab190692 at 1/250 dilution (red) and DAPI nuclear staining (blue). Secondary staining with Goat anti Rabbit IgG (Alexa Flour®555). The image also shows negative control cells.
Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling EDIL3 with ab190692 at 1/340 dilution (red) compared to a Rabbit monoclonal IgGcontrol antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC).
Immunoprecipitation analysis of HeLa cell lysate labeling EDIL3 with ab190692 at 1/50 dilution (lane 1) compared to a PBS control (lane 2). Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.