Anti-eEF1A1 antibody [EPR9470]

• Supplier: Abcam
• Catalog: ab140632
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9470
• Applications: FC IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: EEF1A1
• Conjugate: Unconjugated

Product images

Overlay histogram showing MCF7 cells stained with ab140632 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140632, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-eEF1A1 antibody [EPR9470] (ab140632) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling eEF1A1 with ab140632 at 1/50 dilution.

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eEF1A1 with ab140632 at 1/50 dilution.

Immunofluorescence analysis of MCF-7 cells labelling eEF1A1 with ab140632 at 1/100 dilution.