All lanes : Anti-eEF1A1 antibody - N-terminal (ab135581)Lane 1 : Y79 cell line lysatesLane 2 : T47D cell line lysatesLysates/proteins at 35 µg per lane.developed using the ECL technique
ICC/IF image of ab135581 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135581 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling eEF1A1 with ab135581. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
Flow cytometry analysis of NCI-H292 cells labelling eEF1A1 (green) with ab135581 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.