Anti-EEF2 antibody
| Name | Anti-EEF2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33208 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human, Chicken, Hamster, Bovine, Xenopus, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human EEF2 |
| Description | Rabbit Polyclonal |
| Gene | EEF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-EEF2 antibody (ab33208) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ICC/IF image of ab33208 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33208, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
Hypoxia integration in the serological proteome analysis unmasks tumor antigens - Hypoxia integration in the serological proteome analysis unmasks tumor antigens
Grandjean M, Sermeus A, Branders S, Defresne F, Dieu M, Dupont P, Raes M, De Ridder M, Feron O. PLoS One. 2013 Oct 10;8(10):e76508.
Calcium/calmodulin-dependent protein kinase II mediates group I metabotropic - Calcium/calmodulin-dependent protein kinase II mediates group I metabotropic
Mockett BG, Guevremont D, Wutte M, Hulme SR, Williams JM, Abraham WC. J Neurosci. 2011 May 18;31(20):7380-91.