Anti-EGFR (phospho Y1173) antibody [9H2]
| Name | Anti-EGFR (phospho Y1173) antibody [9H2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24912 |
| Prices | $381.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 9H2 |
| Applications | WB IP ELISA FC ICC/IF ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Dog, Human |
| Antigen | Phosphopeptide conjugate (Human) |
| Blocking Peptide | EGFR (phospho Y1173) peptide |
| Description | Mouse Monoclonal |
| Gene | EGFR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing A431 cells stained with ab24912 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24912, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12169_ab24912-2-ab24912FC.jpg)
Overlay histogram showing A431 cells stained with ab24912 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24912, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-EGFR (phospho Y1173) antibody [9H2] (ab24912) at 0.5 µg/mlLane 1 : Whole cell extracts of control (co) A549 tumor cellsLane 2 : Whole cell extracts of EGF stimulated (EGF) A549 tumor cellsLane 3 : Whole cell extracts of pervanadate treated (VH) A549 tumor cellsLysates/proteins at 20000 cells per lane.SecondaryA HRP-conjugated antibodydeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12170_ab24912_1.jpg)
All lanes : Anti-EGFR (phospho Y1173) antibody [9H2] (ab24912) at 0.5 µg/mlLane 1 : Whole cell extracts of control (co) A549 tumor cellsLane 2 : Whole cell extracts of EGF stimulated (EGF) A549 tumor cellsLane 3 : Whole cell extracts of pervanadate treated (VH) A549 tumor cellsLysates/proteins at 20000 cells per lane.SecondaryA HRP-conjugated antibodydeveloped using the ECL technique
Product References
Characterization of the netrin/RGMa receptor neogenin in neurogenic regions of - Characterization of the netrin/RGMa receptor neogenin in neurogenic regions of
Bradford D, Faull RL, Curtis MA, Cooper HM. J Comp Neurol. 2010 Aug 15;518(16):3237-53.
ErbB-2 signaling is involved in regulating PSA secretion in androgen-independent - ErbB-2 signaling is involved in regulating PSA secretion in androgen-independent
Lee MS, Igawa T, Yuan TC, Zhang XQ, Lin FF, Lin MF. Oncogene. 2003 Feb 6;22(5):781-96.