Anti-Egr1 antibody
| Name | Anti-Egr1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54966 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: SPVATSYPSP VTTSYPSPAT TSYPSPVPTS FSSPGSSTYP SPVHSGFPSP SVATTYSSVP PAFPAQVSSF PSSAVTNSFS ASTGLSDMTA TFSPRTIEIC , corresponding to amino acids 444-544 of Human Egr1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | EGR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Egr1 antibody (ab54966)Lane 1 : EGR1 transfected lysateLane 2 : non-transfected
Egr1 antibody (ab54966) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
![Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12293_Egr1-Primary-antibodies-ab54966-2.jpg)
Overlay histogram showing HeLa cells stained with ab54966 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54966, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Egr1 expression is induced following glatiramer acetate immunotherapy in rodent - Egr1 expression is induced following glatiramer acetate immunotherapy in rodent
Bakalash S, Pham M, Koronyo Y, Salumbides BC, Kramerov A, Seidenberg H, Berel D, Black KL, Koronyo-Hamaoui M. Invest Ophthalmol Vis Sci. 2011 Nov 21;52(12):9033-46.
A shared gene expression signature in mouse models of EBV-associated and - A shared gene expression signature in mouse models of EBV-associated and
Bieging KT, Fish K, Bondada S, Longnecker R. Blood. 2011 Dec 22;118(26):6849-59.
Functional recovery after delayed treatment of ischemic stroke with melanocortins - Functional recovery after delayed treatment of ischemic stroke with melanocortins
Giuliani D, Ottani A, Minutoli L, Stefano VD, Galantucci M, Bitto A, Zaffe D, Altavilla D, Botticelli AR, Squadrito F, Guarini S. Brain Behav Immun. 2009 Aug;23(6):844-50.