![All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilutionLane 1 : untreated 293T cell lysateLane 2 : 293T cell lysate treated with 20% FBSLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12301_Egr1-Primary-antibodies-ab133695-1.jpg)
All lanes : Anti-Egr1 antibody [EPR5014(2)] (ab133695) at 1/1000 dilutionLane 1 : untreated 293T cell lysateLane 2 : 293T cell lysate treated with 20% FBSLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

ab133695 (red), at 1/10, staining Egr1 in permeabilized NIH/3T3 cells treated with 20% FBS using flow cytometric analysis. The green curve corresponds to a Rabbit IgG (negative control).

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD