Anti-EHHADH antibody (ab72795) at 1/500 dilution + Human liver tissue lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-EHHADH antibody (ab72795) at 1/500 dilutionLane 1 : EHHADH transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of fixed and permeabilised HeLa cells using purified ab72795 at 10µg/ml followed by a fluorescein-conjugated secondary antibody.