All lanes : Anti-eIF2A antibody [3A7A8] (ab181770) at 1/500 dilutionLane 1 : MCF7 cell lysateLane 2 : PC12 cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLane 5 : COS7 cell lysateLane 6 : K562 cell lysateLane 7 : Jurkat cell lysateLane 8 : A431 cell lysateLane 9 : NIH 3T3 cell lysate
All lanes : Anti-eIF2A antibody [3A7A8] (ab181770) at 1/500 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, transfected with eIF2A (aa 448-576)-hIgGFc
Anti-eIF2A antibody [3A7A8] (ab181770) at 1/500 dilution + Human eIF2A (aa 448-576) recombinant protein
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181770 at 1/200 dilution followed by DAB staining.
Flow Cytometric analysis of HepG2 cells labeling eIF2A with ab181770 at 1/200 dilution (green) compared to a negative control (red).
Immunofluorescent analysis of HepG2 cells labeling eIF2A with ab181770 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.