Anti-eIF2B epsilon antibody

• Supplier: Abcam
• Catalog: ab32713
• Prices: $382.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon
• Description: Rabbit Polyclonal
• Gene: EIF2B5
• Conjugate: Unconjugated

Product images

All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/500 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

All lanes : Anti-eIF2B epsilon antibody (ab32713) at 1/250 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 6 : Brain (Rat) Tissue Lysate - normal tissue Lane 7 : Liver (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab32713 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32713, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ICC/IF image of ab32713 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32713, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Chronic heart failure reduces Akt phosphorylation in human skeletal muscle: - Chronic heart failure reduces Akt phosphorylation in human skeletal muscle:

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Resistance exercise decreases eIF2Bepsilon phosphorylation and potentiates the - Resistance exercise decreases eIF2Bepsilon phosphorylation and potentiates the

Glover EI, Oates BR, Tang JE, Moore DR, Tarnopolsky MA, Phillips SM. Am J Physiol Regul Integr Comp Physiol. 2008 Aug;295(2):R604-10. doi:

Evidence of placental translation inhibition and endoplasmic reticulum stress in - Evidence of placental translation inhibition and endoplasmic reticulum stress in

Yung HW, Calabrese S, Hynx D, Hemmings BA, Cetin I, Charnock-Jones DS, Burton GJ. Am J Pathol. 2008 Aug;173(2):451-62.