Anti-eIF3e antibody
| Name | Anti-eIF3e antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36766 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB IP |
| Species Reactivities | Chicken, Human, Zebrafish, Mouse, Rat, Bovine, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eIF3e |
| Blocking Peptide | Human eIF3e peptide |
| Description | Rabbit Polyclonal |
| Gene | EIF3E |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-eIF3e antibody (ab36766) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human eIF3e peptide (ab36902) at 1 µg/mlLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) with Human eIF3e peptide (ab36902) at 1 µg/mlLane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) with Human eIF3e peptide (ab36902) at 1 µg/mlLane 8 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) with Human eIF3e peptide (ab36902) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditio
![eIF3e was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF3e and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36766.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 48kDa: eIF3e.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12708_eIF3e-Primary-antibodies-ab36766-2.jpg)
eIF3e was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF3e and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36766.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 48kDa: eIF3e.
ICC/IF image of ab36766 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36766, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
IHC image of ab36766 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36766, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-eIF3e antibody (ab36766) at 1/1000 dilutionLane 1 : Chicken tectum lysate Lane 2 : Zebrafish lysateLysates/proteins at 5 µg per lane.SecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution
Product References
Int6/eIF3e is essential for proliferation and survival of human glioblastoma - Int6/eIF3e is essential for proliferation and survival of human glioblastoma
Sesen J, Cammas A, Scotland SJ, Elefterion B, Lemarie A, Millevoi S, Mathew LK, Seva C, Toulas C, Moyal EC, Skuli N. Int J Mol Sci. 2014 Jan 29;15(2):2172-90.
Mutant human FUS Is ubiquitously mislocalized and generates persistent stress - Mutant human FUS Is ubiquitously mislocalized and generates persistent stress
Acosta JR, Goldsbury C, Winnick C, Badrock AP, Fraser ST, Laird AS, Hall TE, Don EK, Fifita JA, Blair IP, Nicholson GA, Cole NJ. PLoS One. 2014 Jun 9;9(6):e90572.