Anti-EIF3S4 antibody [EPR16147]

• Supplier: Abcam
• Catalog: ab192601
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16147
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EIF3S4 aa 200-300
• Description: Rabbit Monoclonal
• Gene: EIF3G
• Conjugate: Unconjugated

Product images

All lanes : Anti-EIF3S4 antibody [EPR16147] (ab192601) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : MOLT4 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-EIF3S4 antibody [EPR16147] (ab192601) at 1/1000 dilutionLane 1 : mouse Raw 264.7 cell lysateLane 2 : rat PC12 cell lysateLane 3 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling EIF3S4 with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling EIF3S4 using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.