Anti-eIF4G1 antibody
| Name | Anti-eIF4G1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2609 |
| Prices | $391.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Monkey, Mouse, Rabbit, Horse, Hamster, Bovine, Cat, Dog, Chimpanzee, Monkey, Ape, Hamster, Orangutan, Elephant |
| Antigen | Synthetic peptide (Human) - which represents a portion of human eukaryotic translation InitiationFactor 4 Gamma 1 encoded in part by exons 10 and 11 |
| Description | Rabbit Polyclonal |
| Gene | EIF4G1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2609 (4µg/ml) staining eIF4G1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the intestinal cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab2609 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2609, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Mammalian Staufen 1 is recruited to stress granules and impairs their assembly. - Mammalian Staufen 1 is recruited to stress granules and impairs their assembly.
Thomas MG, Martinez Tosar LJ, Desbats MA, Leishman CC, Boccaccio GL. J Cell Sci. 2009 Feb 15;122(Pt 4):563-73.
.
Poly(rC) binding proteins and the 5' cloverleaf of uncapped poliovirus mRNA - Poly(rC) binding proteins and the 5' cloverleaf of uncapped poliovirus mRNA
Kempf BJ, Barton DJ. J Virol. 2008 Jun;82(12):5835-46.
Poliovirus 2A(Pro) increases viral mRNA and polysome stability coordinately in - Poliovirus 2A(Pro) increases viral mRNA and polysome stability coordinately in
Kempf BJ, Barton DJ. J Virol. 2008 Jun;82(12):5847-59.
Tuberous sclerosis complex proteins 1 and 2 control serum-dependent translation - Tuberous sclerosis complex proteins 1 and 2 control serum-dependent translation
Bilanges B, Argonza-Barrett R, Kolesnichenko M, Skinner C, Nair M, Chen M, Stokoe D. Mol Cell Biol. 2007 Aug;27(16):5746-64. Epub 2007 Jun 11.
Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite - Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite
Vary TC, Deiter G, Goodman SA. Am J Physiol Heart Circ Physiol. 2005 Jan;288(1):H121-8. Epub 2004 Sep 23.
Dual inhibition of mTOR and estrogen receptor signaling in vitro induces cell - Dual inhibition of mTOR and estrogen receptor signaling in vitro induces cell
Boulay A, Rudloff J, Ye J, Zumstein-Mecker S, O'Reilly T, Evans DB, Chen S, Lane HA. Clin Cancer Res. 2005 Jul 15;11(14):5319-28.
A microtiter plate assay for assessing the interaction of eukaryotic initiation - A microtiter plate assay for assessing the interaction of eukaryotic initiation
Kimball SR, Horetsky RL, Jefferson LS. Anal Biochem. 2004 Feb 15;325(2):364-8.
IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, - IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability,
Lang CH, Frost RA, Svanberg E, Vary TC. Am J Physiol Endocrinol Metab. 2004 Jun;286(6):E916-26. Epub 2004 Jan 28.
Glucagon represses signaling through the mammalian target of rapamycin in rat - Glucagon represses signaling through the mammalian target of rapamycin in rat
Kimball SR, Siegfried BA, Jefferson LS. J Biol Chem. 2004 Dec 24;279(52):54103-9. Epub 2004 Oct 19.