All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/10000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-eIF4GII antibody - N-terminal (ab155482) at 1/5000 dilutionLane 1 : Mouse JC whole cell lysateLane 2 : Mouse BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human AGS xenograft tissue, labeling eIF4GII using ab155482 at a 1/500 dilution.