IHC image of Embigin Homolog staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127692, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab127692, at a dilution of 1/500, staining Embigin homolog in methanol-fixed A431 cells by Immunofluorescence. The lower image was co-stained with Hoechst 33342.
All lanes : Anti-Embigin homolog antibody (ab127692) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : Jurkat whole cell lysateLysates/proteins at 30 µg per lane.