Anti-Emerin antibody
| Name | Anti-Emerin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54996 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-111 of Human Emerin |
| Description | Mouse Monoclonal |
| Gene | EMD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab54996 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54996, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/13418_ab54996-3-ab54996FC.jpg)
Overlay histogram showing HeLa cells stained with ab54996 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54996, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Emerin antibody (ab54996) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.
Product References
Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 - Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4
Zhuang X, Semenova E, Maric D, Craigie R. J Biol Chem. 2014 Jan 10;289(2):1119-27.
Functional recovery after delayed treatment of ischemic stroke with melanocortins - Functional recovery after delayed treatment of ischemic stroke with melanocortins
Giuliani D, Ottani A, Minutoli L, Stefano VD, Galantucci M, Bitto A, Zaffe D, Altavilla D, Botticelli AR, Squadrito F, Guarini S. Brain Behav Immun. 2009 Aug;23(6):844-50.