Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-EML4 antibody (ab85834) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgdeveloped using the ECL technique
Immunoprecipitation/ Western Blot of ab85834Lane 1: ab85834 at 3µg/mg whole cell lysate.Lane 2: Control IgG.ab85834 at 0.4µg/ml for WB.Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.Chemiluminescence with an exposure time of 30 seconds.