All lanes : Anti-EN2 antibody - C-terminal (ab176010) at 1/1000 dilutionLane 1 : Mouse cerebellum lysateLane 2 : Mouse brain lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling EN2 with ab176010 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using ab176010 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.