Flow cytometric analysis of Hl-60 cells (bottom histogram) compared to a negative control cell (top histogram) using ab176006 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling EN2 with ab176006 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-EN2 antibody - C-terminal (ab176006) at 1/1000 dilution + NCI-H460 lysate at 35 µg