Anti-Endo G antibody

• Supplier: Abcam
• Catalog: ab9647
• Prices: $391.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: GGPRGPGELAKYGLP , corresponding to amino acids 55-70 of Human Endo G
• Blocking Peptide: Human Endo G peptide
• Description: Rabbit Polyclonal
• Gene: ENDOG
• Conjugate: Unconjugated

Product images

Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with anti-EndoG at 2 µg/ml.

ab9647 at 1/50 dilution staining Endo G in human pancreas by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded sections).

ICC/IF image of ab9647 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Endonuclease G mediates alpha-synuclein cytotoxicity during Parkinson's disease. - Endonuclease G mediates alpha-synuclein cytotoxicity during Parkinson's disease.

Buttner S, Habernig L, Broeskamp F, Ruli D, Vogtle FN, Vlachos M, Macchi F, Kuttner V, Carmona-Gutierrez D, Eisenberg T, Ring J, Markaki M, Taskin AA, Benke S, Ruckenstuhl C, Braun R, Van den Haute C, Bammens T, van der Perren A, Frohlich KU, Winderickx J, Kroemer G, Baekelandt V, Tavernarakis N, Kovacs GG, Dengjel J, Meisinger C, Sigrist SJ, Madeo F. EMBO J. 2013 Nov 27;32(23):3041-54.

Protection of cisplatin cytotoxicity by an inactive cyclin-dependent kinase. - Protection of cisplatin cytotoxicity by an inactive cyclin-dependent kinase.

Hodeify R, Megyesi J, Tarcsafalvi A, Safirstein RL, Price PM. Am J Physiol Renal Physiol. 2010 Jul;299(1):F112-20. doi:

Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in - Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in

Zhao ST, Chen M, Li SJ, Zhang MH, Li BX, Das M, Bean JC, Kong JM, Zhu XH, Gao TM. BMC Neurosci. 2009 Sep 8;10:113.

Prediction of localization and interactions of apoptotic proteins. - Prediction of localization and interactions of apoptotic proteins.

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Age-related activation of mitochondrial caspase-independent apoptotic signaling - Age-related activation of mitochondrial caspase-independent apoptotic signaling

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BUB1 mediation of caspase-independent mitotic death determines cell fate. - BUB1 mediation of caspase-independent mitotic death determines cell fate.

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Ceramide synthase is essential for endonuclease-mediated death of renal tubular - Ceramide synthase is essential for endonuclease-mediated death of renal tubular

Basnakian AG, Ueda N, Hong X, Galitovsky VE, Yin X, Shah SV. Am J Physiol Renal Physiol. 2005 Feb;288(2):F308-14. Epub 2004 Oct 12.

Age-related differences in apoptosis with disuse atrophy in soleus muscle. - Age-related differences in apoptosis with disuse atrophy in soleus muscle.

Leeuwenburgh C, Gurley CM, Strotman BA, Dupont-Versteegden EE. Am J Physiol Regul Integr Comp Physiol. 2005 May;288(5):R1288-96. Epub 2005 Jan