All lanes : Anti-Endo G antibody (ab66561) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Spinal Cord (Mouse) Tissue LysateLane 3 : Rat Kidney Tissue LysateLane 4 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : Brain (Rat) Tissue Lysate - normal tissueLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.