Immunoprecipitation of HeLa whole cell lysate using ab191173 (lane 1) at 6 µg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of Endoglycan utilised ab191173 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.
All lanes : Anti-Endoglycan antibody (ab191173) at 1 µg/mlLane 1 : HeLa whole cell lysate prepared using RIPA lysis bufferLane 2 : 293T whole cell lysate prepared using RIPA lysis bufferLane 3 : Jurkat whole cell lysate prepared using RIPA lysis bufferLysates/proteins at 50 µg per lane.developed using the ECL technique