Immunofluorescent analysis of A549 cells labeling Endothelin 1 with ab170544 at 1/10 (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry analysis in formalin fixed, paraffin embedded Human liver tissue labeling Endothelin 1 with ab170544 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
All lanes : Anti-Endothelin 1 antibody - C-terminal (ab170544) at 1/100 dilutionLane 1 : ZR 75 1 cell lysateLane 2 : U251 cell lysateLane 3 : MDA MB453 cell lysateLane 4 : A549 cell lysateLysates/proteins at 35 µg per lane.developed using the ECL technique