Anti-Endothelin B Receptor antibody
| Name | Anti-Endothelin B Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39960 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Endothelin B Receptor |
| Description | Rabbit Polyclonal |
| Gene | EDNRB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab39960 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39960, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Endothelin B Receptor antibody (ab39960) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ab39960 staining human placenta sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab39960 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
Product References
Endothelin receptor expression in idiopathic pulmonary arterial hypertension: - Endothelin receptor expression in idiopathic pulmonary arterial hypertension:
Hall SM, Davie N, Klein N, Haworth SG. Eur Respir J. 2011 Oct;38(4):851-60.
Endothelin-1 inhibits prolyl hydroxylase domain 2 to activate hypoxia-inducible - Endothelin-1 inhibits prolyl hydroxylase domain 2 to activate hypoxia-inducible
Spinella F, Rosano L, Del Duca M, Di Castro V, Nicotra MR, Natali PG, Bagnato A. PLoS One. 2010 Jun 21;5(6):e11241.
CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric - CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric
Taghizadeh R, Noh M, Huh YH, Ciusani E, Sigalotti L, Maio M, Arosio B, Nicotra MR, Natali P, Sherley JL, La Porta CA. PLoS One. 2010 Dec 22;5(12):e15183.