All lanes : Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : Human spleen lysateLane 4 : Fetal heart lysateLane 5 : Fetal muscle lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution + immunoprecipitation pellet from fetal muscle lysateSecondaryHRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG