All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilutionLane 1 : Fetal brain lysateLane 2 : Rat heart lysateLane 3 : Mouse heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution
Flow cytometric analysis of K562 cells labeling ENSA with ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.