All lanes : Anti-ENT1 antibody (ab135756) at 1/1000 dilutionLane 1 : Human Kidney LysateLane 2 : Human Plasma LysateLane 3 : Mouse Kidney LysateLane 4 : Mouse Plasma LysateLane 5 : Rat Kidney LysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H+L conjugated HRP at 1/5000 dilution
ab135756 staining ENT1 in Human colorectal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween 20 and blocked with Protein Block Serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in 0.1M Sodium Citrate (82mls 0.1M) + Citric Acid (18mls 0.1 M) + Elga water (pH6). Samples were incubated with primary antibody (1/500 in Antibody Diluent) for 20 hours at 20°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ICC/IF image of ab135756 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135756 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-ENT1 antibody (ab135756) at 1/50 dilution + Mouse brain tissue lysate at 35 µg