Anti-ENT2 antibody

• Supplier: Abcam
• Catalog: ab48595
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human ENT2
• Description: Rabbit Polyclonal
• Gene: SLC29A2
• Conjugate: Unconjugated

Product images

All lanes : Anti-ENT2 antibody (ab48595) at 1 µg/mlLane 1 : Heart (Mouse) Tissue LysateLane 2 : Kidney (Mouse) Tissue LysateLane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 5 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab48595 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48595, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Overlapping high-resolution copy number alterations in cancer genomes identified - Overlapping high-resolution copy number alterations in cancer genomes identified

Chen CF, Hsu EC, Lin KT, Tu PH, Chang HW, Lin CH, Chen YJ, Gu DL, Lin CH, Wu JY, Chen YT, Hsu MT, Jou YS. Hepatology. 2010 Nov;52(5):1690-701.