All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution (purified)Lane 1 : Untreated HEK293 cell lysateLane 2 : HEK293 cell lysate treated with pervanadateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution (unpurified)Lane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD