All lanes : Anti-Eph receptor A4 antibody (ab5396) at 1/1000 dilutionLane 1 : HeLa Cell LysateLane 2 : NCI-H460 Cell LysateLane 3 : Mouse NIH3T3 Cell LysateLane 4 : Human ovary tissue LysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H+L HRP conjugated at 1/10000 dilution
ab5396 at 1/500 staining rat cerebellar granual cells by ICC/IF. The cells were permeabilized with 0.1% Triton X-100, paraformaldehyde fixed and blocked with 2% serum prior to incubation with the antibody for 1 hour. A Cy3 conjugated goat anti-rabbit was used as the secondary.See Abreview
Immunohistochemical staining of Eph in Human breast carcinoma sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab5396 at a dilution of 1/25 . Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
