Anti-Eph receptor B2 antibody
| Name | Anti-Eph receptor B2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5418 |
| Prices | $390.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P ELISA WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to amino acids 118-133 of Human Eph B2 |
| Description | Rabbit Polyclonal |
| Gene | EPHB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Eph receptor B2 antibody (ab5418) at 1/100 dilution + mouse heart tissue lysate at 0.01 µgdeveloped using the ECL technique
Human cancer tissue stained with ab5418 followed by peroxidase-conjugated secondary antibody and AEC.
ICC/IF image of ab5418 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5418, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Kaposi's sarcoma-associated herpesvirus interacts with EphrinA2 receptor to - Kaposi's sarcoma-associated herpesvirus interacts with EphrinA2 receptor to
Chakraborty S, Veettil MV, Bottero V, Chandran B. Proc Natl Acad Sci U S A. 2012 May 8;109(19):E1163-72. doi:
Identifying transient protein-protein interactions in EphB2 signaling by blue - Identifying transient protein-protein interactions in EphB2 signaling by blue
Darie CC, Deinhardt K, Zhang G, Cardasis HS, Chao MV, Neubert TA. Proteomics. 2011 Dec;11(23):4514-28.
Eph receptor tyrosine kinases regulate astrocyte cytoskeletal rearrangement and - Eph receptor tyrosine kinases regulate astrocyte cytoskeletal rearrangement and
Puschmann TB, Turnley AM. J Neurochem. 2010 May;113(4):881-94.