Anti-EPO Receptor antibody [MM-0031-6G7]
| Name | Anti-EPO Receptor antibody [MM-0031-6G7] |
|---|---|
| Supplier | Abcam |
| Catalog | ab56310 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | MM-0031-6G7 |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Human recombinant EPO Receptor (extracellular domain) |
| Description | Mouse Monoclonal |
| Gene | EPOR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using ab56310 (Proteinase K pretreatment is required).
![Overlay histogram showing K562 cells stained with ab56310 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56310, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/14536_EPO-Receptor-Primary-antibodies-ab56310-3.jpg)
Overlay histogram showing K562 cells stained with ab56310 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56310, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Erythropoietin receptor signaling is membrane raft dependent. - Erythropoietin receptor signaling is membrane raft dependent.
McGraw KL, Fuhler GM, Johnson JO, Clark JA, Caceres GC, Sokol L, List AF. PLoS One. 2012;7(4):e34477.
Productive parvovirus B19 infection of primary human erythroid progenitor cells - Productive parvovirus B19 infection of primary human erythroid progenitor cells
Chen AY, Kleiboeker S, Qiu J. PLoS Pathog. 2011 Jun;7(6):e1002088.
Prognostic impact of erythropoietin expression and erythropoietin receptor - Prognostic impact of erythropoietin expression and erythropoietin receptor
Rades D, Setter C, Dahl O, Schild SE, Noack F. Int J Radiat Oncol Biol Phys. 2011 Jun 1;80(2):499-505. doi:
Erythropoietin Receptor Signaling Through STAT3 Is Required For Glioma Stem Cell - Erythropoietin Receptor Signaling Through STAT3 Is Required For Glioma Stem Cell
Cao Y, Lathia JD, Eyler CE, Wu Q, Li Z, Wang H, McLendon RE, Hjelmeland AB, Rich JN. Genes Cancer. 2010 Jan 1;1(1):50-61.