Flow cytometric staining of BOSC23 cells transiently transfected with an expression vector encoding either EPO Receptor (red curve) or an irrelevant protein black curve (control transfectant) labeling EPO receptor with ab180791 at 1/200 dilution. A PE conjugated secondary antibody was used. A positive signal was obtained only with EPO receptor transfected cells.
Capillary Gel Electrophoresis (CGE) analysis of purified ab180791 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 μg of purified ab180791. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).