Anti-ERG antibody [EPR3864]
| Name | Anti-ERG antibody [EPR3864] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92513 |
| Prices | $404.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3864 |
| Applications | WB IHC-P ICC/IF IHC-F IHC ChIP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ERG aa 450 to the C-terminus |
| Description | Rabbit Monoclonal |
| Gene | ERG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab92513 staining ERG in Human brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 3% Horse serum) for 15 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
![Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution + Jurkat cell lysate at 10 µgSecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/14978_ERG-Primary-antibodies-ab92513-1.jpg)
Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution + Jurkat cell lysate at 10 µgSecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution
![Lane 1 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (blocked with 5% BSA)Lane 2 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (blocked with 5% BSA)Lane 3 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (blocked with 3% milk)Lane 4 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (blocked with 3% milk)Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/14979_ab92513-212876-WBYI123005CS1.jpg)
Lane 1 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (blocked with 5% BSA)Lane 2 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (blocked with 5% BSA)Lane 3 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (blocked with 3% milk)Lane 4 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (blocked with 3% milk)Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab92513 at 1/100 dilution staining ERG in formalin-fixed, paraffin-embedded Human prostate adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining.
Immunohistochemical analysis of Fus A5 transgenic mouse prostate tissue, staining ERG with ab92513. Tissue sections were blocked in 20% goat serum, 2% BSA in PBS (GSB) for 40 min at room temperature, followed by an overnight incubation in primary antibody (1/25 in GSB) at 4°C. Following washes, the sections were incubated with a fluorescently tagged secondary antibody for 30 min at room temperature.
Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab92513 showing -ve staining.
Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using ab92513 showing -ve staining.
Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using ab92513 showing +ve staining.
Immunohistochemical analysis of paraffin embedded normal Human prostate tissue using ab92513 showing -ve staining.
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab92513 showing -ve staining.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD
Product References
Therapeutic targeting of BET bromodomain proteins in castration-resistant - Therapeutic targeting of BET bromodomain proteins in castration-resistant
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Establishment and characterization of a highly tumorigenic African American - Establishment and characterization of a highly tumorigenic African American
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Molecular profiling of ETS gene rearrangements in patients with prostate cancer - Molecular profiling of ETS gene rearrangements in patients with prostate cancer
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TMPRSS2:ERG blocks neuroendocrine and luminal cell differentiation to maintain - TMPRSS2:ERG blocks neuroendocrine and luminal cell differentiation to maintain
Mounir Z, Lin F, Lin VG, Korn JM, Yu Y, Valdez R, Aina OH, Buchwalter G, Jaffe AB, Korpal M, Zhu P, Brown M, Cardiff RD, Rocnik JL, Yang Y, Pagliarini R. Oncogene. 2014 Sep 29.
Celastrol suppresses tumor cell growth through targeting an AR-ERG-NF-kappaB - Celastrol suppresses tumor cell growth through targeting an AR-ERG-NF-kappaB
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Identification of novel TMPRSS2:ERG mechanisms in prostate cancer metastasis: - Identification of novel TMPRSS2:ERG mechanisms in prostate cancer metastasis:
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ETS factors reprogram the androgen receptor cistrome and prime prostate - ETS factors reprogram the androgen receptor cistrome and prime prostate
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ERG expression in mucinous prostatic adenocarcinoma and prostatic adenocarcinoma - ERG expression in mucinous prostatic adenocarcinoma and prostatic adenocarcinoma
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