Anti-ERG antibody [EPR3864(2)]

Name	Anti-ERG antibody [EPR3864(2)]
Supplier	Abcam
Catalog	ab133264
Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR3864(2)
Applications	ICC/IF ICC/IF WB IP IHC-P
Species Reactivities	Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERG aa 450 to the C-terminus (C terminal)
Description	Rabbit Monoclonal
Gene	ERG
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/2000 dilution (purified)Lane 1 : Jurkat cell lysateLane 2 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human colonic carcinoma with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded rat kidney with purified ab133264 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit H+L (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescence staining of MCF7 cells with purified ab133264 at a working dilution of 1 in 500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133264 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

ab133264 (purified) at 1/30 immunoprecipitating ERG in HEK293 cells (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

All lanes : Anti-ERG antibody [EPR3864(2)] (ab133264) at 1/1000 dilution (unpurified)Lane 1 : Fli1 recombinant protein Lane 2 : ERG recombinant protein Lysates/proteins at 0.01 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded human prostate adenocarcinoma tissue labelling ERG with ab133264 at a dilution of 1/100.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Androgen receptor CAG repeat polymorphism and risk of TMPRSS2:ERG-positive - Androgen receptor CAG repeat polymorphism and risk of TMPRSS2:ERG-positive

Yoo S, Pettersson A, Jordahl KM, Lis RT, Lindstrom S, Meisner A, Nuttall EJ, Stack EC, Stampfer MJ, Kraft P, Brown M, Loda M, Giovannucci EL, Kantoff PW, Mucci LA. Cancer Epidemiol Biomarkers Prev. 2014 Oct;23(10):2027-31. doi:

TMPRSS2- driven ERG expression in vivo increases self-renewal and maintains - TMPRSS2- driven ERG expression in vivo increases self-renewal and maintains

Casey OM, Fang L, Hynes PG, Abou-Kheir WG, Martin PL, Tillman HS, Petrovics G, Awwad HO, Ward Y, Lake R, Zhang L, Kelly K. PLoS One. 2012;7(7):e41668.

Expression of ERG, an Ets family transcription factor, identifies ERG-rearranged - Expression of ERG, an Ets family transcription factor, identifies ERG-rearranged

Wang WL, Patel NR, Caragea M, Hogendoorn PC, Lopez-Terrada D, Hornick JL, Lazar AJ. Mod Pathol. 2012 Oct;25(10):1378-83.