Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.
All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilutionLane 1 : Vector only transfected HEK293T cell lysateLane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)developed using the ECL technique
Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582