Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human smooth muscle tissue by immunohistochemistry.
All lanes : Anti-ERGIC1 antibody (ab121574) at 1/250 dilutionLane 1 : RT-4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human plasmaLane 4 : Human liver lysateLane 5 : Human tonsil lysatedeveloped using the ECL technique