Anti-ERK5 antibody [EP791Y]
| Name | Anti-ERK5 antibody [EP791Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40809 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP791Y |
| Applications | WB ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in the C-terminus of Human ERK5 |
| Description | Rabbit Monoclonal |
| Gene | MAPK7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing A549 cells stained with ab40809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40809, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-ERK5 antibody [EP791Y] (ab40809) at 1/5000 dilution + Hela cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/15211_ab40809.jpg)
Anti-ERK5 antibody [EP791Y] (ab40809) at 1/5000 dilution + Hela cell lysate at 10 µg